Antigen | Epitope | Source | Dilution | |
---|---|---|---|---|
Primary | pPERK | pThr981 | Santa Cruz | 1:4000 |
peIF2α | pSer51 | Sigma-Aldrich | 1:2000 | |
AT8 | pSer202/pThr205 | Thermo Scientific | 1:7500 | |
Secondary | goat α rabbit IgG | biotin | Vector Labs | 1:1000 |
goat α rabbit IgG | AlexaFluor 488 | Alexa | 1:500 | |
goat α mouse IgG | AlexaFluor 594 | Alexa | 1:500 |