Figure 6

Association of astrocytes but not microglia with Reelin/CAm deposits. Immunohistochemical analyses involving brain sections obtained from an 82 year-old ND individual. A) Representative image of the fornix labeled with anti-GFAP antibodies and hematoxylin (Ehrlich) staining. Note the GFAP immunoreactivity enclosing the CAm in the dense glial network. Insert represents a Reelin/CAm deposit in an area with low GFAP intensity. B) Double immunofluorescence staining with anti-Reelin antibody ( 142, red, B’) and anti-GFAP antibody ( green, B”). Nuclei are visualized with DAPI-enriched mounting medium. C) Immunoperoxidase staining using anti-Iba1 antibody and hematoxylin (Ehrlich) counterstaining. The pretreatment of the tissue sections with 10 minute citrate irradiation completely diminishes the CAm labeling (arrowhead). D) Double immunofluorescence staining using anti-Reelin antibody ( 142, red, D’) and anti-Iba1 antibody ( green, D’). Scale bars: A, C = 30 μm; B, D = 10 μm.