Figure 5

Dendritic and synaptic proteins in the aged human hippocampus: Differential association with CAm. Immunoperoxidase-hematoxylin staining of brain sections obtained from an 85 year-old ND individual. A) Representative images of the CA2 subfield and fornix (A’) labeled with the dendritic cytoskeletal marker anti-MAP2. Note the strong immunoreactivity in pyramidal cell bodies and dendrites, as well as CAm (A’). B- C) Immunoperoxidase staining using anti-Synapsin-1 (B) and anti-Synaptophysin antibodies (C) depicting the CA2 pyramidal cell layer (B, C), the dentate gyrus molecular layer (B’, C’) and the fornix (B”, C”). While a characteristic synaptic immunoreactivity of both markers was evident in the hippocampus proper and dentate gyrus, no accumulation of these synaptic proteins was seen in CAm. Scale bars: A- C = 30 μm, A’- C’ = 30 μm.