AD-relevant proteins are present in CAm. Representative images of double immunofluorescence labeling involving brain sections obtained from an 82 year-old ND individual counterstained with the nuclear dye DAPI (blue). Red channels show the anti-Reelin ( antibody 142; A, C, D, E), anti-N-APP (B), and anti-PHF Tau (paired helical filament, F) stainings. Green channels depict the anti-Aβ1–40/42
(A’- B’), anti-α-Synuclein (C’, F’), anti Tau (D’) and anti-pTau (E’) signals. Merged channels are shown in A”-F”. Inserts in E’ and F highlight neurofibrillary tangles in CA1 region stained with anti-pTau and anti-PHF Tau antibodies, respectively. Scale bars = 10 μm.