Figure 3

Double-label immunofluorescence of class-III-β-tubulin and Zip14 in a normal control DRG and a DRG of an FA patient. (a)-(c) normal control; (d)-(f) FA; (a) and (d) class-III-β-tubulin (Alexa488, green); (b) and (e) Zip14 (Cy3, red); (c) and (f) merged images. The normal DRG (a) shows large neurons that yield intense class-III-β-tubulin reaction product in perikaryon and dendrites. Zip14 staining (b) is heterogeneous, with less reaction product in the nerve cell marked by “N”. Anti-Zip14 also visualizes satellite cells (b). In FA, a small class-III-β-tubulin-reactive neuron (d) continues to display Zip14 reaction product but is surrounded by multiple layers of Zip14-reactive satellite cells (e). Bars, 20 μm.