Immunofluorescence detection of ASMA and FITC-OVA in uninfected Tg44+/+ mice at 30 min after stereotaxic microinjection of tracer. ASMA is observed as red color and FITC-OVA tracer is green. (a) Epifluorescence, (b-e) Confocal optical sections of 0.38 μm thickness. (a) Most tracer is associated with capillaries (arrows), but some tracer is associated with a larger venule (arrowhead). (b) ASMA-positive artery with associated tracer. (c) Two ASMA-positive arteries with associated tracer. (d) ASMA-negative tracer-positive vein (arrowhead) with 25 μm diameter and two smaller tracer-positive capillaries (arrows) for comparison. (e) ASMA-negative tracer-positive vein (17 μm diameter). Bars: 100 μm (a), 40 μm (b) and 20 μm (c-e).