Figure 4

Immunohistochemical observations of CCR2 protein in spinal cord ventral horns from G1H+/− mice sacrified at onset stage (12 w). Localization of CCR2 immunoreactivity is verified by comparison with that of immunoreactivities for NeuN-immunoreactive (b) neurons, GFAP-immunoreactive (e, h) astrocytes, and Iba1-immunoreactive (k) and CD11b-immunoreactive (n) microglia. CCR2 immunoreactivity is detected with the two different antibodies sc-6228 (a, d, j, m) and PA1-27409 (g), respectively. Panels (c, f, i, l, o) indicate merged images in two other panels of each line. Immunoreactive signals are detected by the double-labeled immunofluorescence method using secondary antibodies conjugated with Cy3 (red) or FITC (green). Scale bar indicates 50 μm (a-o).