Immunohistochemical (a), immunoblot (b) and densitometric (c) analyses for CCR2 protein in the spinal cord of SJL and G1H+/− mice sacrificed at presymptomatic (9 w), onset (12 w) and postsymptopatic (15 w) stages. Immunoreaction product deposits are visualized by the avidin-biotin-immunoperoxidase complex method using 3,3’-diaminomenzidine tetrahydrochloride and hematoxylin as the chromogen and counterstain, respectively, by light microscopy. Scale bar indicates 100 μm (a). Electrophoretic mobility (b) and optical density (c) are compared between the postsymptomatic SJL and G1H+/− groups (n = 5 in each group). Two-way ANOVA provides P < 0.05. Posthoc Bonferroni correction provides *P < 0.05 as compared to the SJL group.