Figure 2

Immunohistochemical observations of MCP-1 protein in the spinal cord of SJL and G1H+/− mice sacrificed at presymptomatic (9 w) and postsymptomatic (15 w) stages (n = 3 in each group). Inset indicates a vacuolated neuron. Immunoreaction product deposits are visualized by the avidin-biotin-immunoperoxidase complex method using 3,3’-diaminomenzidine tetrahydrochloride and hematoxylin as the chromogen and counterstain, respectively, by light microscopy. Scale bars indicate 100 μm (panels) and 50 μm (inset).