Bone morphogenetic protein 2 (BMP2) is a potential KDM1A target gene. a Heatmap shows unsupervised clustering of gene expression obtained for the ONS-76 medulloblastoma cell line 72 h after KDM1A knockdown (right) or mock transfection (left) using Affymetrix U133 Plus 2.0 microarrays. Upregulated genes are represented in red and downregulated genes are represented in blue. KDM1A knockdown was verified in the microarray expression analysis (black arrow), and BMP2 was significantly induced (red arrows, p = 6.4 × 10-6 and 5.4 × 10-5 for the two BMP2 HGU133_Plus Affymetrix array probe sets for BMP2, 205289_at and 205290_s_at, respectively). b The significant increase of BMP2 expression upon KDM1A knockdown was confirmed by real-time RT-PCR for ONS-76 cells 72 h after knockdown or mock transfection. *p < 0.05 c KDM1A knockdown increased the level of phosphorylated SMAD5 by 220% detected in western blots of whole-cell lysates of ONS-76 cells 72 h after knockdown or mock transfection. β-actin expression was used as a loading control.