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Table 2 Sequence of primers used in RT-Q-PCR

From: Evidence for new targets and synergistic effect of metronomic celecoxib/fluvastatin combination in pilocytic astrocytoma

Name

Sequence

Size/bp

18S

F : 5’-CTACCACATCCAAGGAAGGCA-3’

71

R : 5’-TTTTTCGTCACTACCTCCCCG-3’

GAPDH

F : 5’-CAAATTCCATGGCACCGTC-3’

101

R : 5’-CCCACTTGATTTTGGAGGGA-3’

β-actin

F : 5’-CCACACTGTGCCCATCTACG-3’

99

R : 5’-AGGATCTTCATGAGGTAGTCAGTCAG-3’

CD36

F : 5'-TGCAAGTCCTGATGTTTCAGA-3'

142

R : 5'-TGGCTTGACCAATAGGTTGAC-3'

IQGAP1

F : 5'-AGAACAGACCAGATACAAGGCGA--3'

97

R : 5'-CTTAGGCAATCCAATCTCATCCA-3'

CRK

F : 5'-GGAGTGATTCTCAGGCAGGA-3'

113

R : 5'-TCCCGGATTCTCAAGATGTC-3'

ICAM1

F : 5'-AGCTTCTCCTGCTCTGCAAC-3'

153

R : 5'-CATTGGAGTCTGCTGGGAAT-3'

COX-2

QUANTITECT (REF :QT00040586)

68

  1. Forward (F)/Reverse (R) primers and size of corresponding amplified fragment for each gene are listed. PCR conditions were as follows: 10 min at 95°C, followed by 45 cycles of 15 s at 95°C, 30 s at 60°C for CD36, ICAM1, IQGAP1, CRK and COX-2, 10 min at 95°C, followed by 30 cycles of 15 s at 95°C, 30 s at 67°C for 18S and 10 min at 95°C, followed by 45 cycles of 15 s at 95°C, 20 s at 65°C for GAPDH and β-actin.
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Acta Neuropathologica Communications

ISSN: 2051-5960

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