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Table 2 Sequence of primers used in RT-Q-PCR

From: Evidence for new targets and synergistic effect of metronomic celecoxib/fluvastatin combination in pilocytic astrocytoma

Name Sequence Size/bp
18S F : 5’-CTACCACATCCAAGGAAGGCA-3’ 71
R : 5’-TTTTTCGTCACTACCTCCCCG-3’
GAPDH F : 5’-CAAATTCCATGGCACCGTC-3’ 101
R : 5’-CCCACTTGATTTTGGAGGGA-3’
β-actin F : 5’-CCACACTGTGCCCATCTACG-3’ 99
R : 5’-AGGATCTTCATGAGGTAGTCAGTCAG-3’
CD36 F : 5'-TGCAAGTCCTGATGTTTCAGA-3' 142
R : 5'-TGGCTTGACCAATAGGTTGAC-3'
IQGAP1 F : 5'-AGAACAGACCAGATACAAGGCGA--3' 97
R : 5'-CTTAGGCAATCCAATCTCATCCA-3'
CRK F : 5'-GGAGTGATTCTCAGGCAGGA-3' 113
R : 5'-TCCCGGATTCTCAAGATGTC-3'
ICAM1 F : 5'-AGCTTCTCCTGCTCTGCAAC-3' 153
R : 5'-CATTGGAGTCTGCTGGGAAT-3'
COX-2 QUANTITECT (REF :QT00040586) 68
  1. Forward (F)/Reverse (R) primers and size of corresponding amplified fragment for each gene are listed. PCR conditions were as follows: 10 min at 95°C, followed by 45 cycles of 15 s at 95°C, 30 s at 60°C for CD36, ICAM1, IQGAP1, CRK and COX-2, 10 min at 95°C, followed by 30 cycles of 15 s at 95°C, 30 s at 67°C for 18S and 10 min at 95°C, followed by 45 cycles of 15 s at 95°C, 20 s at 65°C for GAPDH and β-actin.