Figure 4
From: Promoter DNA methylation regulates progranulin expression and is altered in FTLD
GRN promoter DNA methylation is altered in FTLD-TDP patients. Genomic DNA from human frontal cortex samples was subjected to bisulfite conversion to analyze DNA methylation in the GRN promoter by MassARRAY (See Figure 1b). (a) Box plots show average percentage of DNA methylation in amplicons A-1 to A-5 comparing healthy controls (white), FTLD patients (red), AD patients (blue) and PD patients (yellow). Whiskers depict 5–95 percentile, **p < 0.01, Kruskal Wallis test with Dunn’s Multiple Comparison Test. (b) Negative correlation of average DNA methylation across amplicon A-1 to GRN mRNA expression. Correlation between parameters was quantified by linear regression analysis, r2 and p-values are given. Color code as in (a). (c) Relative GRN mRNA expression levels in brains from controls, FTLD, AD and PD patients quantified by qPCR and normalized to the housekeeping genes PGK1 and GAPDH. Mean ± SD.