Ob/ob mice display classic signs of insulin resistance. A, B) An IPGTT showed significantly elevated blood glucose levels in ob/ob mice throughout the test. The blood glucose of ob/ob mice increased more than 10 mmol/L at its maximal level as opposed to nondiabetic mice that elevated less than 6 mmol/L after glucose injection, indicating severe glucose intolerance in ob/ob mice. C, D) Similar to the IPGTT, data from the ITT showed reduced insulin sensitivity in ob/ob mice. In fact, an insulin dose of 1.5 U/Kg did not decrease the blood glucose level of ob/ob mice, whereas this dose lowered the blood glucose of nondiabetic controls by approximately 3.6 mmol/L. E- G) At 10 weeks of age, ob/ob mice had significantly elevated blood glucose and serum insulin levels. Accordingly, the HOMA-IR measure of insulin resistance was significantly higher in ob/ob mice. ** = p < 0.01, *** = p < 0.001, **** = p < 0.0001. IPGTT n = 7 nondiabetic mice, n = 6 ob/ob. ITT n = 4 nondiabetic mice, n = 4 ob/ob.