Figure 2

A α-Synuclein and ATP13A2 both demonstrated increased insolubility in PD anterior cingulate cortex, with increased ratios of SDS-soluble to TBS-soluble protein. B-G ATP13A2 (red; B, E) and α-synuclein (green; C, F) immunostaining in PD anterior cingulate cortex shows partial colocalization (yellow) in Lewy bodies in some (D) but not all (G) cortical neurons. H-J ATP13A2 immunolabeling of diffuse cytoplasmic puncta in anterior cingulate cortex was not different between neurons from control cases (H) and neurons without Lewy bodies from either pure PD cases (I) or pure DLB cases (J). K-L An increase in the number of ATP13A2 immunolabeled (red) cytoplasmic puncta was observed in neurons containing Lewy bodies (α-synuclein, green; K) compared to neurons without Lewy bodies (L) in anterior cingulate cortex of PD and DLB cases.