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Figure 1 | Acta Neuropathologica Communications

Figure 1

From: ATP13A2 (PARK9) protein levels are reduced in brain tissue of cases with Lewy bodies

Figure 1

Increases in α-synuclein and β-amyloid are correlated with decreases in ATP13A2 protein levels in Lewy body diseases. A Representative Western immunoblots of α-synuclein, ATP13A2 and β-actin in membrane-associated protein fractions from PD and control anterior cingulate cortex. Quantitative data shows membrane-associated α-synuclein was increased 264% and total ATP13A2 was reduced 29% in PD anterior cingulate cortex, but neither α-synuclein nor ATP13A2 levels were significantly changed in PD occipital cortex. Data are shown as a percentage of mean control levels. B Representative Western immunoblots of α-synuclein, ATP13A2 and β-actin in membrane-associated protein fractions from PD, DLB and control parahippocampal cortex . Quantitative data shows membrane-associated α-synuclein was increased by 280% in PD and 314% in DLB compared with controls, and membrane-associated ATP13A2 was reduced from control levels by 39% in PD and 55% in DLB parahippocampal cortex. These protein changes were not significantly different between the PD and DLB cases in this region. Data are shown as a percentage of mean control levels. C Regression analysis revealed significant negative correlations between membrane-associated α-synuclein and both membrane-associated ATP13A2 and total ATP13A2 in anterior cingulate cortex in PD and DLB. Data are shown as a percentage of mean control levels. D Membrane-associated Aβ42 and ATP13A2 were significantly negatively correlated in PD and DLB parahippocamal cortex. Data are shown as a percentage of mean control levels. E Diffuse β-amyloid-positive plaques (asterisk) were present in DLB but not PD anterior cingulate cortex and showed no colocalization or physical association with ATP13A2-positive neurons (arrow).

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