Fig. 4

ACLIP2::MET fusion identified in tumor samples by RNA-seq. Both tumors showed the CLIP2::MET fusion with the same exon-exon fusion junctions. The fusion occurs in-frame, resulting in the expression of a fusion protein encoded by the 5’ portion of the CLIP2 gene (exons 1–11 out of a total of 17 exons) and the 3’ portion of the MET gene (exons 15–21 out of a total of 21 exons), which contains the protein kinase domain of MET. B DNA methylation results for both cases. A t-SNE map includes Case 1, Case 2, reference samples comprising low-grade glioma, subclass midline pilocytic astrocytoma (LGG, PA MID), diffuse leptomeningeal glioneuronal tumor (DLGNT), anaplastic pilocytic astrocytoma (ANA PA), low-grade glioma, subclass hemispheric pilocytic astrocytoma and ganglioglioma (LGG, PA GG ST), low-grade glioma, methylation class control tissue, reactive tumor microenvironment (CONTR, REACT), and dysembryoplastic neuroepithelial tumor (LGG, DNT). Case 1 clusters with the LGG, PA GG ST reference samples, although the tumor received a suggestive score (0.83) for the methylation class DLGNT by the random forest algorithm. Case 2 clusters with the LGG, DNT reference samples, but did not match to any specific methylation class using the random forest algorithm.