Fig. 6

The role of Nrf2 in the cytotoxicity mediated by Panobinostat. A The inhibitory responses of proliferation for different Nrf2 expressions with Panobinostat administration in MMQ and GH3 cells. n = 3; *p < 0.05, **p < 0.01. B The inhibitory responses of PRL and GH hormone secretions for different Nrf2 expressions with Panobinostat administration in MMQ and GH3 cells. n = 3; *p < 0.05, **p < 0.01. C Tumor volume growth curves and tumor weights of nude mice in different treatment groups. n = 5; *p < 0.05, **p < 0.01, ^##p < 0.01. D Left: Representation of immuno-blots for different Nrf2 and NQO1 expressions in different groups. Right: Bar graph of densitometric analysis. n = 3; *p < 0.05, **p < 0.01, ^#p < 0.05, ^##p < 0.01. E Redox changes evidenced by the levels of luminol CL, lucigenin CL, MDA content, and total SOD activity in each group. n = 3; *p < 0.05, **p < 0.01, ^##p < 0.01. F Representative IHC images of H&E, 8-OHdG, Nrf2, NQO1, and caspase-3 staining in different treatment groups. G Representative images of TUNEL staining in mice PitNET specimens. H Representation of immuno-blots for different apoptosis-related protein expressions in different groups