Fig. 3

Transcriptome-wide RNA-sequencing assays to identify on-target ability of Panobinostat in PitNET primary, MMQ, and GH3 cells. A Transcriptome strategy of RNA sequencing conducted on different PitNET cells exposed to Panobinostat for 24 h is shown in schematic diagram. MMQ, GH3, and primary cell groups contained 3 replicates, respectively. B The common core-enriched signaling pathways changed following Panobinostat treatment. C Single GSEA was used to analyze the targeted signaling pathway. Normalized enrichment score (NES) indicated the analysis results across gene sets. D The gene expressions of Nrf2 and its downstream genes in PitNET cell lines following Panobinostat treatment by RNA sequencing