Fig. 4

MSA-activated microglia induce tau phosphorylation in a NLRP3-dependent manner. a The levels of tau phosphorylation in primary neurons treated by MSA, MM and AM. b Quantification of the WB results in a via Image J. c The key protein levels in tau phosphorylation pathway in primary neurons in the presence or absence of CY09. d Quantitative analysis of the WB bands in c using Image J. For b, d, n = 3, representing three independent experiments, data are mean ± SEM. One-way ANOVA with Tukey’s multiple comparisons test for multiple groups in was used for statistical analysis. e, Phosphorylated Tau levels at multiple phosphorylation sites in neurons treated with or without MM. f, Quantitative analysis of the WB bands in (e) using Image J. g, Phosphorylated Tau levels at multiple sites in the primary neurons treated with or without MM detected by ICC (scale bar, 25 μm). h. Quantification of ICC results in (g) by Image J. For f, h, n = 3, representing three independent experiments, data are mean ± SEM, and Student’s test was used for statistical analysis for multiple groups was used for statistical analysis. ns, no significance; *p < 0.05, **p < 0.01, ***p < 0.001and ****p < 0.0001