Fig. 1

Quantification of Iba1 positive cells across different brain regions of control and disease groups. a Panel for immunohistochemical Iba1 staining of microglia for Control, MIX, AD and LBD cases in hippocampus (HPC), frontal cortex (FC), occipital cortex (OCC) and substantia nigra (SN). Microglia were quantified in 10 μm-thick sections stained with Iba1 antibody. b–e Quantification of Iba1 positive microglia in HPC, FC, OCC and SN. HPC data points contain hippocampal subregions (CA4-1, subiculum, entorhinal cortex, see Supp. Figure 2), OCC contains data points from subregions (striate cortex and peristriate cortex). Red – female, blue – male. No sex differences could be shown across the examined brain regions. One-way ANOVA with Tukey's multiple comparisons test was used for statistical analysis. *p < 0.05, **p < 0.01, ***p < 0.001. Scale bar = 60 μm