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Fig. 5 | Acta Neuropathologica Communications

Fig. 5

From: Dysregulation of neuroprotective lipoxin pathway in astrocytes in response to cytokines and ocular hypertension​

Fig. 5

Regulation of macroglia reactivity in response to ocular hypertension. (A) Representative immunofluorescence images of GFAP (green) at 2 weeks, and (B) GFAP (green) RBPMS (red) at 8 weeks of ocular hypertension. Nuclei were labeled with DAPI. All images were obtained using a 40x objective. Scale bar = 50 μm. Labels: ganglion cell layer (GCL), inner nuclear layer (INL), outer nuclear layer (ONL). (C) Representative images of a portion of flat-mounted retinas stained with GFAP (green) at 8 weeks. (D) Muller glia reactivity gene, GLAST, was analyzed by qPCR at 2 and 4 weeks. The results were normalized to GAPDH. (E) Astrocyte markers were significantly upregulated at 2, 4, and 8 weeks when analyzed by qPCR. The results were normalized to GAPDH and controls. n = 4 (2 retinas were pooled for each replicate). *p < 0.05, **p < 0.01, **p < 0.001. (Ctrl- Control; OHT- ocular hypertension)

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Acta Neuropathologica Communications

ISSN: 2051-5960

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