Table 1 Primary antibodies used for histology and immunofluorescence
From: CNS-associated macrophages contribute to intracerebral aneurysm pathophysiology
Markers | Detection | Species | Dilution | Manufacturer | Cat. number |
|---|---|---|---|---|---|
Mouse samples | |||||
α-SMA | α-smooth muscle actin | Mouse | 1:500 | Abcam | ab8211 |
CD206 | CAMs | Rat | 1:500 | BioRad | MCA2235GA |
CD3 | T-lymphocytes | Rabbit | 1:200 | Abcam | ab5690 |
CD31 | Endothelial cells | Rabbit | 1:500 | Abcam | ab28364 |
CD62P | P-selectin | Rat | 1:500 | BP Pharmingen | 553742 |
CD68 | Lysosomes | Rat | 1:500 | Abcam | ab53444 |
Col IV | Blood vessels | Goat | 1:1000 | SouthernBiotech | 1340-01 |
Fibrinogen | Fibrinogen | Goat | 1:1000 | Non-commercial | |
H3Cit | Neutrophil extracellular traps | Rabbit | 1:500 | Abcam | ab5103 |
Iba1 | Microglia | Goat | 1:1000 | Abcam | ab5076 |
Laminin | Blood vessels | Rabbit | 1:1500 | Abcam | ab11575 |
Ly6G | Neutrophils | Rat | 1:500 | StemCell | 60031 |
MPO | Myeloperoxidase | Rabbit | 1:500 | Abcam | ab9535 |
P2Y12R | Microglia | Rabbit | 1:500 | Anaspec Inc | as55043A |
Human samples | |||||
CD68 | Lysosomes | Mouse | 1:500 | Dako | M0814 |
MHCII | MHCII molecules | Mouse | 1:500 | Santa Cruz | sc-53302 |
CD11b | Monocytes/macrophages, granulocytes, natural killer cells | Mouse | 1:50 | Santa Cruz | sc-1186 |
CD163 | CAMs | Rabbit | 1:50 | Novusbio | NBP3-08325B |