Fig. 1

A new mouse model of IAs at the middle cerebral artery. a Schematic representation of experimental design and the timeline scale. b Representative images of formation, growth and rupture assessed by longitudinal MRI. Contrast-enhanced T1 weighted (CE-T1-w) MRI was used to confirm the presence of IAs (yellow arrow). T2*-w MRI was performed to confirm the rupture of IAs with the presence of hemorrhage (red arrow). Once IA ruptures, it is no longer observable with T1-w MRI, bleeding appears as hypointense signal on T2*-w MRI. c Representative T1-w images, with the location of the IA at the lateral part of the brain. Three-dimensional IA reconstruction created from the MR scans revealed the saccular type of IAs. d Occurrences of ruptured and unruptured aneurysms at different time points revealed that many aneurysms appear already at the Day 5, and most of them are ruptured by the Day 15 (presented in e). f Kaplan–Meier survival graph. g Average number of IA per animal quantified from T1-w MRI during the 15-days timeline (left), average number of IAs per animal assessed per histological analysis at the end of the protocol (right). Scale bar: 500 µm. h Hemorrhage volumes measured from T2*-w scans during the experimental timeline