Fig. 4

Loss of host-derived GPNMB promotes a pro-inflammatory tumor immune microenvironment in a murine glioma model. A Representative core region staining of CD3 (left), Ki-67 and merge with DAPI (right; CD3 = red, Ki-67 = green, DAPI = blue) of brain slices from tumor-bearing WT and KO. Scale bar represents 20 µm. The graphs (Top left, core, CD3⁺DAPI⁺: WT n = 7, KO n = 7; Top right, invasive edge (IE), CD3⁺DAPI⁺: WT n = 7, KO n = 7; Bottom left, core, CD3⁺Ki-67⁺DAPI⁺: WT n = 7, KO n = 7; Bottom right, IE, CD3⁺Ki-67⁺DAPI⁺: WT n = 7, KO n = 6) show the summary data from WT and KO mice. B Representative core region staining of CD8 (left) and merge with DAPI (right; CD8 = green, DAPI = blue) of brain slices from tumor-bearing WT and KO. Scale bar represents 40 µm. The graphs (left, core: WT n = 7, KO n = 7; right, IE: WT n = 7, KO n = 7) show the summary data from WT and KO mice. C Representative core region staining of Gzmb (left) and merge with DAPI (right; Gzmb = red, DAPI = blue) of brain slices from tumor-bearing WT and KO. Scale bar represents 40 µm. The graphs (left, core: WT n = 7, KO n = 7; right, IE: WT n = 7, KO n = 7) show the summary data from WT and KO mice. D Representative core region staining of Foxp3 (left) and merge with DAPI (right; Foxp3 = red, DAPI = blue) of brain slices from tumor-bearing WT and KO. Scale bar represents 40 µm. The graphs (left, core: WT n = 7, KO n = 7; right, IE: WT n = 7, KO n = 7) show the summary data from WT and KO mice. Statistical analysis was performed using an unpaired t-test. Error bars represent SD. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001