Fig. 7

Discovery of SIRT1 interactions in the autistic brain, linking chromatin remodelers to autophagy. (A) Volcano plot of differentially expressed proteins (DEPs) in the ADNP patient cerebellum, represented by the significance (−log10(p)) and effect size (log2FC). Proteins with a significant downregulation are shown in blue and those with an upregulation in red. Note the marked difference in expression of the ADNP-interacting protein CBX1/HP1β. (B) Immunoblot confirmations of DEPs exposed to their specific antibodies. (C) Top 15-ranked canonical IPA pathways of the ADNP brain, represented as z-scored expression values and significance (−log p-value). Activated pathways are presented in orange, respectively lowered activity in blue; no activity pattern available (grey); pathways with no difference in activity (white). (D) A predictive protein–protein interaction network was generated by String Version 11.5, integrating associations of a co-expression hub identified amongst the DEPs. Proteins are represented by colored network nodes in relation to each other with SIRT1 fulfilling a centralizing role. The edges illustrate functional associations and the lines between the nodes represent the existence of evidence for associations