Fig. 3

Expression of VAPB or PTPIP51 rescues mutant TDP43 induced disruption to synaptic vesicle release. (a and b) Kinetics of FM 4–64 release from synaptic boutons in cortical neurons transfected with (a) EGFP + CTRL, EGFP + TDP43-Q331K + CTRL or EGFP + TDP43-Q331K + either VAPB or PTPIP51 and (b) EGFP + CTRL, EGFP + TDP43-A382T + CTRL or EGFP + TDP43-A382T + either VAPB or PTPIP51 as indicated. Synaptic vesicle release was induced by treatment with KCl and FM 4–64 signals were determined from images acquired by time-lapse microscopy. Representative FM 4–64 traces are shown on the left; bar charts show F/F0 FM 4–64 fluorescent signals 5 s post KCl treatment. Data were analysed by one-way ANOVA with Tukey’s post hoc test. N = 68–102 boutons in (a) and N = 133–146 boutons in (b) from 3 independent experiments. Error bars are SEM; *** p≤0.001, ns not significant