Fig. 5
Astroglial response to Aβ. A Aβ40 + 42 and GFAP immunostaining of brain tissue from 18-month-old tg-UppSwe, tg-ArcSwe and tg-Swe mice. Co-localization of Aβ and GFAP was lacking in tg-UppSwe mice but was abundant in tg-ArcSwe and tg-Swe mice. Scale bar 100 µm. ELISA quantification of GFAP in TBS (B) and TBS-T (C) brain extracts from 18-months-old wt, tg-UppSwe, tg-ArcSwe and tg-Swe mice. Not significant (ns), **P < 0.01, ***P < 0.001. D Schematic description of the procedure to establish primary astrocyte monocultures originating from the cerebral cortices of embryonic mouse brain. Primary astrocyte cultures were exposed to sonicated, Cy3-labeled fibrils of synthetic AβUpp, AβArc or Aβwt (all Aβ1-42). Cells were stained for GFAP, LAMP-1 and cell nuclei (Dapi). While AβUpp clustered on the surface of cells, AβArc and Aβwt appeared to be phagocytosed to a larger extent. Scale bar: 20 µm