Fig. 2

Aβ neuropathology in tg-UppSwe transgenic mice. A Aβ40 and Aβ42 immunostaining of tg-UppSwe mouse brain sections from different age groups. Magnifications, indicated by red squares, of cerebral cortical (B) and hippocampal (C) Aβ42 staining from 18-month-old tg-UppSwe mice. The blue squares represent the region displayed in the higher magnification images. Scale bar: 200 µm. D Synaptic staining of 70 nm thick serial brain sections from 18-month-old tg-UppSwe mouse stained for pre-synapses (synaptophysin; yellow), post-synapses (PSD95; magenta) and fibrillar Aβ (OC; cyan) with composite images in (IV and VIII). Synaptic staining was qualitatively reduced within plaques (arrows I–IV). Three-dimensional reconstructions (V–VIII) demonstrated Aβ staining within both pre-synapses (arrow, V) and post-synapses (arrow, VI). Panels I-IV show segmented images from a single section and V-VIII show zoomed in 3D reconstructions of five serial sections. Scale bars: 10 µm (I–IV) or 1 µm (V–VIII)