Fig. 8

SCs lacking Gnao1 display more differentiation due to the elevated cAMP levels. Aa1 Gene ontology analysis of the up-regulated differentially expressed genes (DEGs) in the differentiated SCs (Diff-NCs) and Gnao1-KD-SCs compared to controls (NCs). Bar graph showing representative GO categories of those DEGs. Aa2 Heat map showing DEGs function related to myelination. Aa3 KEGG analysis of the up-regulated DEGs in Diff-NCs and Gnao1-KD-SCs compared to NCs. Bubble diagram showing representative KEGG categories of those DEGs. Bb1 WB analysis of Adcy2 in Gnao1-KD-SCs and controls (NCs), as shown are representative WB images (up) and the statistical histogram (bottom). T-test, n = 3, **, p < 0.01 vs NCs. Bb2 The levels of cAMP in Gnao1-KD-SCs and NCs were detected by enzyme-linked immunosorbent assay (ELISA). T-test, n = 3, **, p < 0.01 vs NCs. Bb3 Differentiation of Gnao1-KD-SCs and controls treated with db-cAMP free SC in vitro differentiation medium. WB analysis showing Gnao1-KD-SCs expressed higher level of MAG compared with NCs, indicating that Gnao1-KD-SCs could differentiate even in the absence of db-cAMP. Schematic display experimental method (left), also shown are representative WB images and quantitative statistics (right). T-test, n = 3, **, p < 0.01 vs NCs