Fig. 5

Gnao1 knockdown in SCs inhibited proliferation of SCs seeded on PDL but did not affect the proliferation of SCs seeded on fasciculated DRG axons. A The proliferation rate of Gnao1-shRNA or scramble-shRNA transfected SCs cultured on PDL are shown in the representative ICC images (left) and statistical analyses (right). In the ICC images, green dots (EdU positive), the proliferating SCs; blue dots (Hoechst), the nucleus, scale bar = 100 μm. Zoomed in is the magnification of the white box, scale bar = 100 μm. Histogram shows that Gnao1 knockdown significantly decreases SC proliferation. T-test, n = 9, ***, p < 0.0001 vs Scramble. B The proliferation rate of Gnao1-shRNA or scramble-shRNA transfected SCs cultured on fasciculated DRG axons are shown in the representative ICC images (left) and statistical analyses (right). In the ICC images, green dots (EdU positive), the proliferating SCs; blue dots (Hoechst), the nucleus, scale bar = 100 μm. Zoomed in is the magnification of the white box, scale bar = 100 μm. Histogram shows that Gnao1 knockdown did not affect SC proliferation. T-test, n = 4–5, Ns, p > 0.05 vs Scramble, no statistical difference