Fig. 4

Knockdown Gnao1 expression in spinal cord neurons did not affect re-myelination after sciatic nerve injury. A The expression of MAG in the regenerated nerves of Gnao1-SKD mice and controls (Ctrl) was detected by WB. As shown are representative WB images (up) and the statistical histogram (bottom). T-test, n = 3, Ns, p > 0.05 vs control, no statistical difference. B IHC images of regenerative nerves from Gnao1-SKD mice and controls, MAG for regenerative myelin-associated protein (red), scale bar = 500 μm. Zoomed in is the enlargement of the white box area, scale bar = 100 μm. The histogram is a quantitative statistic of the amount of myelin sheaths per unit area. T-test, n = 3, Ns, p > 0.05 vs control, no statistical difference. C Representative transmission electron microscopy (TEM) images of the regenerated sciatic nerve of Gnao1-SKD mice and controls after 21 day injury, showing the myelin sheath structure (left), scale bar = 5 μm. Zoomed in is the magnification of the red box, scale bar = 500 nm. The distribution map of the g-ratio of myelin sheath of the regenerated nerve in Gnao1-NKD mice and controls and the diameter of the regenerated axon wrapped by myelin sheath (middle top), and the histogram are the quantitative statistics of g-ratio (upper right) myelin thickness, lamellar number, axon diameter and myelin density (lower right). T-test, n = 3, Ns, p > 0.05 vs control, no statistical difference. D CatWalk gait analysis performed at 4, 7, 10, 13, 17 and 20 days post-injury, the left histograms showing the values of SFI and representative footprints of the left hind paw (injured side) of Gnao1-SKD mice and controls are showed on the right. T-test, n = 3, Ns, p > 0.05 vs control, no statistical difference