Fig. 2

Knockdown Gnao1 expression in in SCs of sciatic nerve promotes myelin re-formation after nerve injury. A The expression of MAG in the regenerated nerves of Gnao1-NKD mice and controls (Ctrl) was detected by WB. As shown are representative WB images (up) and the statistical histogram (bottom). T-test, n = 3, *, p < 0.05 vs control. Bb1 IHC images of regenerative nerves from Gnao1-NKD mice and controls, MAG for regenerative myelin-associated protein (red), GFP for viral expression (green), scale bar = 500 μm. Zoomed in is the enlargement of the white box area, scale bar = 100 μm. Bb2 The histogram is a quantitative statistic of the amount of myelin sheaths per unit area. T-test, n = 3, **, p < 0.01 vs control. C Representative transmission electron microscopy (TEM) images of the regenerated sciatic nerve of Gnao1-NKD mice and controls after 21 day injury, showing the myelin sheath structure (left), scale bar = 5 μm. Zoomed in is the magnification of the red box, scale bar = 500 nm. The distribution map of the g-ratio of myelin sheath of the regenerated nerve in Gnao1-NKD mice and controls and the diameter of the regenerated axon wrapped by myelin sheath (middle top), and the histogram are the quantitative statistics of g-ratio (upper right) myelin thickness, lamellar number, axon diameter and myelin density (lower right). T-test, n = 3, Ns, p > 0.05 vs control, no statistical difference. *, p < 0.05, ***, p < 0.001 vs control. D CatWalk gait analysis performed at 1, 4, 7, 10, 13, 17 and 21 days post-injury, the left histograms showing the values of sciatic function index (SFI) and representative footprints of the left hind paw (injured side) of Gnao1-NKD mice and controls are showed on the right. T-test, n = 3, Ns, p > 0.05 vs control, no statistical difference. *, p < 0.05, **, p < 0.01 vs control