Fig. 7

IFNγ is produced primarily by CD4+ T cells. A Male and female 8–12-week-old Thy1.1/IFNγ reporter mice were injected with either Olig001-GFP or Olig001-SYN in the dorsal lateral striatum. 4 weeks post-transduction, tissue was harvested for neuroinflammation. B A panel of representative immunohistochemistry images of CD4+ T cells (CD4), CD8+ T cells (CD8), NK cells (NK1.1), astrocytes (GFAP), and microglia (Iba1) with the IFNγ reporter Thy1.1. C Percentages of CD45+ cells generated from flow cytometry data looking at Thy1.1+ (IFNγ producing) cells. D Dot plots between Olig001-GFP and Olig001-SYN injected Thy1.1/IFNγ reporter mice showing Thy1.1+ cells in lymphocytes (CD45+ , CD11b−). Mean values are plotted ± SEM, non-parametric Wilcoxon, *p < 0.05. E Flow cytometry dot plots of Thy1.1+ TCRb+ T cells (CD45+ , CD11b−, TCRb+ , Thy1.1 +). Mean values are plotted ± SEM, non-parametric Wilcoxon test, *p < 0.05. F Flow cytometry showing Thy1.1+ CD4+ T cells (CD45+ , CD11b−, TCRb+ , CD4+ , Thy1.1 +). Mean values are plotted ± SEM, non-parametric Wilcoxon test, **p < 0.01. For immunohistochemistry experiments, n = 3 per group. For flow cytometry experiments n = 3 (two mouse striatum tissues pooled per n) per group