Fig. 5
Neutralizing IFNγ activity attenuates neuroinflammation. A Three days prior to Olig001-SYN transduction, both WT female and male 8–12 week old mice received an i.p. injection of 200ng of anti-IFNγ (XMG1.2) or its isotype control (IgG1). Three days later, Olig001-SYN was transduced into the striatum and received another treatment. Afterwards mice received follow up treatments every three days for 30 days. After the 30 days, tissue was collected to assess for neuroinflammation. B Dot plots displaying the overall myeloid populations (CD45+ , CD11b +) and lymphocytes (CD45+ , CD11b−). Below are dot plots of microglia (CD45mid, CD11b +) and monocytes/macrophages (CD45hi, CD11b +). Mean values ± SEM are plotted, non-parametric Wilcoxon test, ns = no significance, *p < 0.05. C Representative immunohistochemistry of Iba1 (green) and MHCII (red) positive cells within the striatum and corpus collosum. D flow cytometry of mononuclear TCRb+ (CD45+ . CD11b−, TCRb +), CD4+ (CD45+ , CD11b−, TCRb +) and CD8+ T cells (CD45+ , CD11b−, TCRb +). Mean values ± SEM are plotted, non-parametric Wilcoxon *p < 0.05. E representative immunohistochemistry images of CD4+ and CD8+ T cells (red) and insoluble α-syn, pSer129 (green) in the striatum. Scale bars are at 10uM. For immunohistochemistry experiments, n = 3 per group. For flow cytometry experiments n = 4 (two mouse striatum tissues pooled per n) per group