Fig. 4

Tbet -/- mice are protected against neuronal loss and demyelination in the presence of α-syn GCI pathology. A WT and Tbet -/- were injected bilaterally with either Olig001-GFP or Olig001-SYN into the dorsal striatum. Pathology developed over 6 months, and then tissue was collected for unbiased stereology and luxol fast blue staining. The contours on the tissue cross section define the area of striatum that was quantified for both stereology and luxol fast blue. B Representative images of NeuN+ stains used in unbiased stereology analysis. The red boxes highlight the area where the zoom insert (top right) was taken from. Quantification of the unbiased stereology is to the right of the representative images. Mean values ± SEM are plotted, two-way ANOVA with Tukey post hoc for significance, *p < 0.05. C Representative images of the luxol fast blue stain used to assess for demyelination. The red boxes highlight the area where the zoom insert (bottom left) was taken from. Quantification is to the right of images. Mean values ± SEM are plotted, student t-test, *p < 0.05. For unbiased stereology and luxol fast blue, n = 7–9 per group. Each data point represents one mouse