Fig. 1

Tbet -/- attenuates myeloid responses in the Olig001-SYN mouse model of MSA. A Tbet -/- mice or WT mice 8–12 weeks of age received bilateral (flow cytometry) or unilateral (immunohistochemistry) stereotaxic injections of Olig001-SYN into the dorsal lateral striatum. 4 weeks post-injection, tissue was collected to assess for neuroinflammation. B Flow cytometry on isolated striatal tissues, displaying both myeloid (CD45+ , CD11b) and lymphocyte populations (CD45+ , CD11b−) (top); the resident microglia (CD45lo, CD11b +) and monocytes/macrophages (CD45hi, CD11b +) (bottom). C The quantification of monocytes/macrophages (CD45hi, CD11b +) and infiltrating monocytes (CD45hi, CD11b+ , Ly6C +) isolated from the striatum with flow cytometry. Mean values ± SEM are plotted, non-parametric Wilcoxon test, *p < 0.05, ***p < 0.001. D Quantification of flow cytometry showing the percent of microglia within the striatum and their MHCII expression. Mean values ± SEM are plotted, non-parametric Wilcoxon test, ns = no significance, *p < 0.05. E Representative images of 3,3’Diaminobenzidine (DAB) staining and quantification of mean gray value of the MHCII expression in the dorsal lateral striatum. Mean values ± SEM are plotted, non-parametric Wilcoxn test, ***p < 0001. F Representative images depicting MHCII expression (red) on activated microglia (Iba1, green) in the dorsal lateral striatum. Scale bars are at 50uM. For immunohistochemistry experiments, n = 3 per group. For flow cytometry experiments to n = 5 (two mouse striatum tissues pooled per n) per group