Fig. 2

Representative images of RNAscope combined with immunofluorescence in Lewy body disease cases. A A neuron without α-synuclein (α-syn) immunoreactivity (IR) in the substantia nigra, which has numerous SNCA and neuron-specific RBFOX3 transcripts in the nucleus and cytoplasm. B Punctate α-syn IR (arrow) in a neuron, which shows many fine dots-like α-syn IR, and an irregular-shaped compact inclusion (arrowhead), which shows compact α-syn IR without a core or halo. Numerous SNCA transcripts are observed in the neuron containing the punctate α-syn IR compared with the neuron with irregular-shaped compact inclusion. C Brainstem-type Lewy body (bLB) containing a round core and pale halo shows few SNCA transcripts. D. A neuron containing a punctate α-syn IR in the amygdala shows numerous SNCA transcripts. E. A neuron without α-syn IR (arrow) and a cortical LB (cLB, arrowhead) in the amygdala show few SNCA transcripts. F. Astrocytes with (arrowhead) and without (arrows) α-syn IR in the amygdala contain few SNCA transcripts. Each image set represents confocal images taken from the same field of view showing phosphorylated-α-syn (p-Syn, A–E) or 5G4 α-syn (5G4-syn, F) immunostaining (green), SNCA (red), neuron-specific RBFOX3 (A–E) or astrocyte-specific ALDH1L1 (F, magenta), DAPI (blue), and the enlarged merged image. Scale bars represent 20 μm