Fig. 4

IDH2-mutant astrocytoma xenograft model. A Hematoxylin and eosin staining of non-xenograft formed mouse brain (YMG25P, upper) and xenograft formed mouse brain (YMG25R, lower). Inset, high magnification. B Kaplan–Meier curve estimates of mice implanted with xenograft non-forming YMG25P (blue) and forming YMG25R (red). C Sanger sequencing indicating IDH2 (c.515G > A, R172K) and TP53 (c.742C > T, R248W) mutation in YMG25R xenograft. D Unsupervised clustering using t-SNE analysis for YMG25R xenograft. Brain tumor classifier was determined by v11b4. E Copy number profiling of YMG25R xenograft. F Immunohistochemistry for indicated proteins in YMG25R xenograft (upper) and sham mouse brain (lower). Bars, 50 μm