Fig. 2
From: MYC overexpression and SMARCA4 loss cooperate to drive medulloblastoma formation in mice
Loss of SMARCA4 and MYC overexpression cooperate to drive brain tumor formation in vivo. (A) Schematic overview of the cell culture and transplantation protocol for the generation of SMARCA4-deficient MYC-overexpressing tumors. (B) Tumor-free survival of transplanted CD1nu/nu mice; grey area represents the 95% confidence interval. Censored mouse at day 80 had to be sacrificed due to illness unrelated to tumor development. (C) Representative HE staining of tumors in the brains of n = 5 transplanted mice in sagittal brain section. (D,E) High-power HE stainings of distinct areas within the tumors showing (D) anaplastic or (E) apoptotic features. (F) Tumors show complete loss of SMARCA4 in IHC interspersed with SMARCA4-positive blood vessels. (G) PCR using DNA isolated from tumor biopsies confirms Smarca4 recombination on a genetic level. (H-I) Tumors stain positive for (H) GFP and (I) MYC, confirming transduction with the MYC-GFP construct. (J) Tumors are highly proliferative as indicated by Ki67 stainings; (K) with a high degree of apoptosis according to Cleaved Caspase-3 (CC3) signals. (L-O) Tumors show scattered expression of (L) SOX2 and (M) Nestin but no signal for (N) NeuN or (O) OLIG2. Scale bar corresponds to 2 mm in C, to 25 μm in D + F (also applicable to E, H, J-O), and to 50 μm in I