Fig. 1

Dissection of the locus coeruleus noradrenergic neurons→rostral ventromedial medulla (NA^LC→RVM) circuit. A Schematic diagram of injection and coronal sections of the LC. B Coronal section (at − 200 µm location) is shown. M: medial, D: dorsal, L: lateral, V: ventral. C mCherry (mCh) co-localizes with tyrosine hydroxylase (TH) in the LC derived from (A) and (B). Scale bars, 20 μm (B, Left), 50 μm (B, Right), 100 μm (A, Bottom and C)