Fig. 4

CRISPR/Cas9-mediated correction of PSEN2 ^N141I iPS lines. a Schematic showing guide RNAs used in the targeting of CRISPR/Cas9, as well as donor ssODNs utilized to introduce wild-type genotype. b Left 2 panels show GFP positive HEK293T cells indicating Cas9 system with guide RNA expression, NT refers to non-transfected; right 2 panels show sample of GFP positive iPSCs after lipofection with pCas9-gN141I-GFP vector. c Sanger sequencing results from iPSC lines, showing corrections in the N141I mutation. d Aβ 42/40 ratio detected by ELISA in 72 h conditioned media from mutant, control or Cispr-Cas9 corrected BFCNs (DIV 34). n = 4, 4 independent experiments with technical triplicates. *, p < .05; **, p < .01 Student T-test