Fig. 3

Detection of αsyn-associated pathology in AAV1-αsyn mouse. a, b Photomicrographs of representative regions of the brain of AAV1-αsyn injected mice.at 3 months of age. a Phosphorylated αsyn (pS129) was highly increased within the neuronal soma and to a lesser extent within the axonal projections.5G4 immunostaining was less intense but follow the same pattern as pS129. Neither pS129 nor 5G4 were found in AAV-venus animals (bottom line). b Brain sections digested with proteinase K showed PK-resistant αsyn in neuronal cell bodies and neurites with small inclusions (< 10 μm). c Representative Western Blot of Triton-X100 soluble and 2% SDS fraction of 3 month olds animals. Scale bars in a and b = 50 μm