Fig. 1

Olig001-GFP expression in rats. a Representative image of GFP expression in the striatum and corpus callosum of rats 4-weeks following injection of Olig001-GFP. Robust GFP expression is seen in the white matter bundles in the striatum and corpus callosum (n = 4). Scale bar 100 μm. b Stereological quantification estimated 126,574 ± 11,303.77 GFP+ cells, c which occupied a volume of 3.873 ± 0.304 mm³ in the striatum. d GFP expression is seen to colocalize with oligodendroglia marker Olig2 in the corpus callosum (top panel) and striatum (second panel). Very little GFP expression is seen to colocalize with neuronal marker NeuN (third panel) or astrocyte marker GFAP (bottom panel). Scale bar 50 μm. e 94–97% of GFP+ cells in the corpus callosum and striatum co-localized with oligodendrocyte marker Olig2, only 2.9–4.7% of GFP+ cells co-localizing with neuronal marker NeuN, 0.18–0.49% of GFP+ cells co-localizing with astrocyte marker GFAP phenotype [effect of phenotype F(2,9) = 11,711.6, P < 0.0001, oligodendrocytes vs. astrocytes p < 0.0001 Fisher’s PLSD post hoc, oligodendrocytes vs. neurons, p < 0.0001, Fisher’s PLSD, astrocytes vs. neurons p = 0.0001 Fisher’s PLSD]