Fig. 14
Quantification of IHC staining in the cortex for PrPC (a), T-Tau (b), P-Tau (c), GFAP (d), IBA1 (e), MAP2 (f) and MBP (g). Quantification of PrPC and T-Tau was determined as the average staining intensity in the cortex. Semi-quantification of P-Tau staining localized to the injury zone was analyzed using a semi-quantification rating of P-Tau intensity on a scale of 0–2 (2 being maximum staining). Quantification of GFAP, IBA1, MAP2 and MBP was determined as the percentage burden of immunopositive pixels in the cortex. Significant differences between groups were determined using one-way ANOVA with Tukey post-hoc test for multiple comparisons. For all graphs, # indicates significant differences (p < 0.05) between Tga20 sham and Tga20 sCHI; * Shows significant differences (p < 0.05) between WT sCHI and Tga20 sCHI; ‡ Shows significant differences (p < 0.05) between Tga20 sCHI and PrPKO sCHI