Fig. 1

Tau is associated with TNTs in neuronal CAD cells. Cells were plated in Lab-Tek chamber slides and co-infected with LVs encoding mCherry-Actin (red) and V5-hTau1N4R (green). Neuronal CAD cells were processed for immunocytochemistry analysis using anti-V5 antibodies visualized with an Alexa 488-labeled secondary antibody (green) and anti-acetylated-tubulin visualized with an Alexa 647-labeled secondary antibody (white). Nuclei were labeled with DAPI (blue). Cells were imaged with an inverted laser-scanning confocal microscope using a 40× oil-immersion lens (NA 1.3), and the images were processed using ZEN and ImageJ. A focal plane was collected for each specimen. Scale bars: 10 μm. TNTs (white arrows), which are not always bound to the dish, are shown in enlargements