Fig. 2

Cell stress does not accelerate disease onset. a-b Experimental scheme in which rNLS8 animals were weaned and then exposed to repeated cycles of hTDP-43ΔNLS expression (for 2 wks) followed by suppression (for 2 wks) by feeding them chow with or without Dox. This lasted for 3 months in the “young cycling” group (a), or 12 months in the “aged cycling” group (b) prior to removing Dox continuously for 4 wks. c-d Repeated transgene expression and suppression, designated “cycling”, for either 3 months (grey bar) or for a whole year (black bar) does not exacerbate TA denervation (c) or lumbar MN loss (d) at 4 weeks off Dox compared, to young or aged mice that are off Dox without cycling (red and blue bars). Data are mean ± SD (n = 3–4 for all groups of mice)