Fig. 3

Tor1a+/- mice do not present morphological changes in brain and spinal cord after crush injury. a-c Representative images of Nissl stained neurons in (a) somatosensory cortex, (b) striatum and (c) spinal cord of naïve wt, naive Tor1a+/-, wt crush and Tor1a+/- crush injured mice. High magnification inserts of single cells are included in each image. d Representative images of CD11b + microglia in spinal cord. e-g Diagrams showing Nissl + profiles per mm² in (e) cortex and (f) striatum of the respective groups of mice ipsilateral and contralateral to the sciatic nerve crush injury and (g) ipsilateral spinal cord (mean ± SEM). h Number of CD11b + cells per mm² of the different groups of mice in white (wm) and grey matter (gm) are shown (mean ± SEM). n = number of mice are shown below the diagrams. Statistical analysis was performed by using the parametric one-way ANOVA with posthoc Tukey test