Fig. 7From: Marked changes in dendritic structure and spine density precede significant neuronal death in vulnerable cortical pyramidal neuron populations in the SOD1G93A mouse model of amyotrophic lateral sclerosisDecreased apical dendritic spine density of SOD1 LVPNs within the somatosensory cortex commencing from P65-75, compared to WT controls. Images show mosaics of somatosensory cortex LVPNs from P65-75 WT (a) and SOD1 (b) mice. High-magnification images of apical dendrites from P65-75 WT (c), SOD1 (d) and basal dendrites from P120 WT (e) and SOD1 (f) mice somatosensory cortex are also shown. Examples of apical and basal dendritic spines are identified with red arrowheads. g shows a scatterplot quantifying unchanged total dendritic arbor length (μm) of SOD1 LVPNs (grey squared) compared to WT controls (white circles). h shows a scatterplot quantifying unchanged apical arbor length (μm) of SOD1 LVPNs (grey squared) compared to WT controls (white circles). i shows a scatterplot quantifying significantly decreased apical dendritic spine density per 100 μm of SOD1 LVPNs (grey squared) compared to WT controls (white circles) at P65-75 and P120. All data mean ± SEM, with two-way ANOVAs followed by Bonferroni post-tests, *P < 0.05. n = 7, 5, 5 and 8 for WT P8-15, P28-35, P65-75 and P120 respectively. n = 6, 5, 5 and 12 for SOD1 P8-15, P28-35, P65-75 and P120 respectively. Scale bar: b, 50 μm. c and f, 5 μmBack to article page