Fig. 5

LRP1 deficient microglia have an amoeboid morphology. Cx3cr1 ^creER-Lrp1 ^fl/fl and Lrp1 ^fl/fl mice were injected daily with LPS (1 mg/kg) for 4 consecutive days. Brain sections were stained with Iba1 antibody, imaged by confocal microscopy and analyzed with ImageJ. a Representative images of microglia and b corresponding Sholl analyses. *p < 0.05; **p < 0.01; ***p < 0.001, 2-way ANOVA. (Scale bar = 60 μm). c Soma size of the microglia. 10 microglia per animal were quantified for each experimental group. **p < 0.01, Student’s t-test. d Quantification of area covered by Iba1+ microglia per image. *p < 0.05, Student’s t-test. e Numbers of microglia per unit area (n = 3–4 mice per condition). Values represent mean ± s.e.m