Fig. 3

Detection of proliferative cells in intracranial aneurysm lesions of rat. a, Proliferative cells in the small intestine of rats after intraperitoneal injection of EdU (80mg/kg) were detected by click reaction (green color). Merged images with nuclear staining by DAPI are shown. Magnified images are shown in the lower panels. Scale bar = 50μm. b and c, Proliferative cells in intracranial arterial walls of rats before (day 0, b) or after (day 28, c) aneurysm induction were labeled after intraperitoneal injection of EdU. Immunostaining for α-smooth muscle actin (SMA) is shown to visualize the arterial walls. Scale bar = 50μm